1)研发了结构简单、成本低廉的新型漂浮反应器, 在漂浮反应器漂浮支架设计、锚定、水动力特性、大规模产业示范等方面取得了突出的研究成果(Bioresource Technology, 2019, 29, 122018;Journal of Cleaner Production, 2021, 283:124648;Biotechnology for Biofuels, 2019,12,54)。该研究工作为本项目高效、经济光生物反应器的开发和设计提供了新的思路。特别的,该漂浮反应器可以直接漂浮在水产养殖水体表面,可以实现饵料微藻的原位培养与原位利用,实现了微藻水产养殖和微藻养殖的完美结合,且已证明该反应器可以实现饵料微藻的高效、集约化生产。

图1 漂浮反应器在水产养殖应用示意图
2)与博士导师研究团队提出了基于漂浮反应器为基础的贝类-微藻类协同的海洋碳汇技术(图2),微藻在该技术方案中有两个重要的作用,一个是提供贝类生长的食物,另外一个是固定因被贝类贝壳吸收碳酸根而释放的CO2。该技术方案可以实现基于生物碳泵(即微藻光合作用固碳)和碳酸盐泵(贝类贝壳钙化固碳)协同的最大化碳固定,并以此方案以此获得马斯克基金组织的XPRIZE Carbon Removal(碳去除大赛)中国预热赛第四名的成绩;

图2 贝类-微藻协同的海洋碳汇技术示意图
3)开发了一种精准、智能化混合策略(Science of the Total Environment, 2022, 852: 158515;授权中国发明专利:202011104785.9,图3),该策略基于光照强度和培养温度的动态变化,可实现实时智能、精精准地控制开放池的桨轮搅拌速度,从而避免混合能量在非最适生长区间的浪费。结果显示,该混合策略在动态扰动条件下仍具有与恒定的高混合速率对照组相同的螺旋藻生物质生产效率,但其混合能量较对照组减少了约30%。

图 3 开放池的精准、智能化混合策略及其生产效果
4)开发了利用高浓度碳酸氢盐/碳酸盐形成“碳池”的微藻高效碳捕捉和利用技术(Journal of CO2 Utilization, 2020, 37,320-327, 图4),解决了碳传质和碳利用不同步的矛盾,突破了微藻高效利用空气中低浓度二氧化碳的技术瓶颈,为大幅度降低微藻生产成本和利用微藻实现“碳中和”提供了新思路。

图4 用于碳捕捉和微藻培养的人工碳池示意图
1. Chenba Zhu, Shulin Chen, Yu ji, Ulrich Schwaneberg, Zhanyou Chi, Progress toward a bicarbonate-based microalgae production system. Trends in Biotechnology, 2022, 40 (2): 180-193, Cell 子刊,IF:21.942,一区,(连续高被引)
2. Chenba Zhu, Yu ji, Xiang Du, Fantao Kong, Zhanyou Chi, Yunpeng, Zhao, A smart and precise mixing strategy for efficient and cost-effective microalgae production in open ponds. Science of the Total Environment, 2022, 852: 158515, IF: 10.753,一区
3. Zhu, Chenba, Han, Desen, Li, Yonghai, Zhai, Xiaoqian, Chi, Zhanyou, Zhao, Yunpeng, Cai, Haibo, Cultivation of aquaculture feed Isochrysis zhangjiangensis in low-cost wave driven floating photobioreactor without aeration device. Bioresource Technology, 2019, 29, 122018, IF: 11.889,一区
4. Chenba Zhu,Yimei Xi, Xiaoqian Zhai, Jinghan Wang, Fantao Kong, Zhanyou Chi, Pilot Outdoor Cultivation of an Extreme Alkalihalophilic Trebouxiophyte in Floating Photobioreactor using Bicarbonate as Carbon Source. Journal of Cleaner Production, 2021, 283:124648, IF:11.072,一区
5. Chenba Zhu, Jian Che, Xiaoqian, Zhai, Fantao Kong, Jinghan Wang, Zhanyou Chi., Cost-effective and efficient production of carbohydrate from an alkalihalophilic Leptolyngbya sp. in a photobioreactor with periodical mixing. ACS Sustainable Chemistry & Engineering, 2020, 8(40): 15310-15316, IF: 9.224,一区
6. Xiaoqian Zhai, Chenba Zhu (共同一作), Yongcheng Zhang, Hao Pang, Fantao Kong, Jinghan Wang , Zhanyou Chi. Seawater supplemented with bicarbonate for efficient marine microalgae production in floating photobioreactor on ocean: A case study of Chlorella sp. Science of the Total Environment, 2020, 738, 139439, IF: 10.753,一区
7. Chenba Zhu, Zhanyou Chi, Chunwei Bi, Yunpeng, Zhao, Haibo, Cai, Hydrodynamic performance of floating photobioreactors driven by wave energy, Biotechnology for Biofuels, 2019,12,54, IF: 7.670,一区
8. Chenba Zhu, Ruolan Zhang, Longyan Cheng, Zhanyou Chi, A recycling culture of Neochloris oleoabundans in a bicarbonate-based integrated carbon capture and algae production system with harvesting by auto-flocculation, Biotechnology for Biofuels, 2018, 11, 204, SCI IF: 7.670,一区
9. Chenba Zhu, Xiaoqian Zhai, Yimei Xi, Jinghan Wang, Fantao Kong, Yunpeng Zhao, Zhanyou Chi, Efficient CO2 Capture from the Air for High Microalgal Biomass Production by a Bicarbonate Pool. Journal of CO2 Utilization, 2020, 37, 320-327, IF:8.321,二区
10. Chenba Zhu, Xiaoqian Zhai, Jinghan Wang, Desen Han, Yonghai Li, Yimei Xi, Yajie Tang, Zhanyou Chi, Large-scale cultivation of Spirulina in a floating horizontal photobioreactor without aeration or an agitation device, Applied Microbiology and Biotechnology, 2018, 102, 8979-8987, IF: 5.560,二区